Next-generation sequencing-based transcriptome analysis of Cryptolaemus montrouzieri under insecticide stress reveals resistance-relevant genes in ladybirds
نویسندگان
چکیده
منابع مشابه
Transcriptome analysis using next-generation sequencing.
Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript leve...
متن کاملNext Generation Sequencing Based Transcriptome Analysis of Septic-Injury Responsive Genes in the Beetle Tribolium castaneum
Beetles (Coleoptera) are the most diverse animal group on earth and interact with numerous symbiotic or pathogenic microbes in their environments. The red flour beetle Tribolium castaneum is a genetically tractable model beetle species and its whole genome sequence has recently been determined. To advance our understanding of the molecular basis of beetle immunity here we analyzed the whole tra...
متن کاملDe Novo Sequencing-Based Transcriptome and Digital Gene Expression Analysis Reveals Insecticide Resistance-Relevant Genes in Propylaea japonica (Thunberg) (Coleoptea: Coccinellidae)
The ladybird Propylaea japonica (Thunberg) is one of most important natural enemies of aphids in China. This species is threatened by the extensive use of insecticides but genomics-based information on the molecular mechanisms underlying insecticide resistance is limited. Hence, we analyzed the transcriptome and expression profile data of P. japonica in order to gain a deeper understanding of i...
متن کاملNext-Generation Sequencing Reveals One Novel Missense Mutation in COL1A2 Gene in an Iranian Family with Osteogenesis imperfecta
Background: Osteogenesis imperfecta (OI) is a clinically and genetically heterogeneous disorder characterized by bone loss and bone fragility. The aim of this study was to investigate the variants of three genes involved in the pathogenesis of OI. Methods: Molecular genetic analyses were performed for COL1A1, COL1A2, and CRTAP genes in an Iranian family with OI. The DNA samples were analyzed by...
متن کاملtranscriptome from next generation sequencing data
Motivations Recent data coming from the comparison of genomes of different individuals in human species and of different genotypes in plants has led interesting findings about the differences among individuals, ecotypes or genotypes. Cross-species conservation analysis revealed that many of the genes potentially encoded by novel sequences are conserved across a number of mammal and might be bio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genomics
سال: 2012
ISSN: 0888-7543
DOI: 10.1016/j.ygeno.2012.05.002